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AMPTS II - methane potential analysis tool

The AMPTS II is the analytical tool preferred by scientists and engineers for conducting various anaerobic batch fermentation tests. This includes performing biochemical methane potential (BMP) tests for substrates, anaerobic biodegradability studies, specific methanogenic activity (SMA) assays, as well as conducting residual gas potential (RGP) analyses on digested slurry. All of this is performed with easy access to sampling, analysis, recording, and report generation, fully integrated and automated.

  • Highly precise and accurate data
  • Significant reductions in time and labour
  • Standardisation of measurement procedures, data interpretation and reports
  • User-friendly operations with remote access
Article no: 01-0000-02



Determine the true bio-methane potential

The Automatic Methane Potential Test System (AMPTS) II allows users to conduct anaerobic toxicity assays and determine the true biochemical methane potential (biomethane potential) and dynamic degradation profile of any biomass substrate.

This in turn will allow users to more easily determine the optimal retention time and mix of substrates for co-digesting, screen proper pre-treatment methods, and evaluate the need for additives.


Significantly reduce your labour demands

The AMPTS II is a well engineered analytical device developed for on-line measurements of ultra low biomethane and biogas flows produced from the anaerobic digestion of any biological degradable substrate at laboratory scale.

The automated analytical procedure significantly reduces labour demands for carrying out methane potential (biomethane potential) tests when compared to traditional methods or competitive solutions on the market.

Standardise and compare results

The AMPTS II is a universal platform for all anaerobic biodegradability, methane potential (BMP) and specific methanogenic activity (SMA) test protocols, allowing for the standardisation of measurement procedures, data interpretation and reports.

This allows for methane potential (biomethane potential) and toxicity assay data from different laboratories around the world to be easily compared, thus creating value over and above the high quality results obtained from operating the AMPTS II.


Get access to highly precise and accurate data

The real-time performance and outstanding features of the AMPTS II satisfies the highest demands for data accuracy and precision with regards to methane potential (biomethane potential) tests and anaerobic toxicity assays. This high quality data can be used to extract important kinetic information of the degradation process, which in turn provides for a much better understanding of the dynamic degradation behaviour of a specific substrate.

Outstanding real-time performance

The AMPTS II is an efficient analytical instrument for conducting real-time BMP, anaerobic biodegradability and SMA assays – having sampling, analysis, recording and report generation fully integrated and automated.

A multi-channel analyser consisting of 15 parallel reactors and the same number of gas flow meters (flow cells) attached to a data acquisition system, allows for the real-time investigation of a high number of samples. This unique design makes the AMPTS II an extremely precise and accurate instrument, and with a wide range of applications.

Temperature and pressure compensation

The real-time temperature and pressure compensation feature of the AMPTS II ensures that the impact of measurement conditions can be minimised and data presentation standardised.

The temperature and pressure of gas are measured every time a flow cell opens, allowing the user to derive exact kinetic information compensated for any variation over time, while considering the vapour content of the gas. The normalised volumes are presented under dry conditions at 0 °C and 1 atm.

Automatic removal of gas overestimation

Methane gas overestimation arises after flushing the system with an inert gas such as nitrogen. Consequently, carbon dioxide in the newly formed biogas is mistakenly counted as methane gas. This can lead, in some cases, to an overestimation of methane gas production by as much as 20–30%, and even more depending on the volume of the headspace. The AMPTS II calculates and removes this overestimation, providing for more accurate and reliable data on methane gas production.

Network ready and easy access

The AMPTS II is designed to allow easy access from a remote location. Through the use of standard protocols and connections, the AMPTS II behaves like any other unit on an internal network, secured by a user definable password.

All interactions with the software are conducted through a web browser using any computer. Thus, experiment monitoring can be carried out with iPhone or iPad mobile devices, systems running on the Android platform, or Windows phones.

Stable and reliable operation

The internal software of the AMPTS II runs on an embedded ARM CPU utilising the Linux operating system. This provides for an unmatched stability, data protection and minimum downtime. Thus, users will never experience data loss under normal operating conditions, which is always a risk with PC based solutions. Software updates issued by Bioprocess Control can be installed by the user in a controlled and easy manner.

A software application designed for methane potential tests

The AMPTS web-based software application is a natural extension of an already universal hardware platform. It has been specially designed for carrying out methane potential, specific methanogenic activity and anaerobic biodegradability tests.

This easy to understand application enables users to set-up an experiment, monitor its progress and download the results with little effort. Moreover, all data is in a format that allows for easy analysis.

Total control throughout an experiment

The Control feature of the AMPTS software application lets users manage both the mixing of reactors and status of each batch test in real-time during an ongoing experiment. Users can control the speed and on/off time of mechanical agitation to ensure that each reactor is operated under optimum mass transfer conditions.

The Control feature also allows users to easily start, pause and stop data acquisition of an ongoing experiment. Overall, users are given optimal control of all reactors and batch experiments at all times with the simple click of a virtual button from the software user interface.

See your experiment in real-time and anywhere

With the Graph feature and embedded web server, users can see their experiment in real-time and from anywhere. Users can also monitor the accumulated gas volume and flow rate of each reactor by selecting and viewing only the reactor they wish to see.

All values displayed are already adjusted for gas volumes normalized to 1 atm, 0 °C and zero moisture content. Moreover, if a flush gas with a different gas composition from biogas is used for establishing an anaerobic condition, the impact of the flush gas is also taken care of.

Sample incubation unit

Maximum number of reactors per system: 15
Reactor material: glass
Standard reactor volume: 500 ml
Dimension of unit: 53 x 33 x 24 cm
Temperature control: up to 95 °C (203 °F) (precision of 0.2 °C)
Mixing in the reactor: Multifunctional agitation system with Brushless DC Motor for long-life operation. Remote speed and on/off control via AMPTS II software control interface. Manually adjustable direction and time interval, as well as timer function to set time periods for reversal. Modular design for low maintenance. Rotating speed range: 10-200 rpm.


Carbon dioxide absorption unit

Carbon dioxide trap bottles: 15
Volume of carbon dioxide trap bottles: 100 ml
Dimension of unit: 44 x 30 x 6 cm
Absorption liquid: 3 M NaOH with pH indicator, 80 ml per bottle (not included)
Absorption efficiency: >98%


Flow cell array and DAQ unit

Working principle: liquid displacement and buoyancy Up to 15 cells running in parallel
Built-in pressure and temperature sensor
Measuring resolution: 10 ml Detection capacity: up to 13 l cumulative gas per channel for each batch test
Measuring range for instant gas flow rate: 10 to 120 ml/min Integrated data acquisition (maximum capacity 2×10^4 flow cell openings)
Dimension: 51 x 44 x 18 cm
Housing: plastic
Repeatability: ±1%


Software & System

  • A software application specially designed for biogas potential and anaerobic biodegradability tests
  • Web based software running on an embedded server
  • On-line real-time gas flow and volume display
  • Automatic real-time pressure and temperature compensation
  • Real-time gas flow and volume normalisation
  • Algorithm to avoid overestimation of gas flow and volume (that may be introduced by flush gas during experiment setup)
  • Possibility of multiplexing, allowing for simultaneous batch analysis at different startup times
  • User friendly guidelines for experiment setup
  • On-line system logger for operational diagnosis
  • Power supply: 12 V DC / 5A
  • Usage: indoor 

For more information, see Demo or User Manual

What Customers Say?

AMPTS helped us minimising the differences in laboratory skills between different researchers by following the same procedure for BMP testing in which manual handling is minimised, while a huge number of data points are gathered… By using AMPTS apparatus we can achieve reproducible results even with students who perform the test for the first time. We now include the AMPTS as a standardised test in our regular curriculum practical work.

Prof. Jules van Lier Delft University of Technology, the Netherlands

We use AMPTS II for both sales process and technical process optimization. The biomethane potential test performed with AMPTS II can provide useful data to demonstrate benefits of Cambi thermal hydrolysis technology for sludge pre-treatment before anaerobic digestion. This allows our clients to evaluate the possible return of investment on implementation of Cambi thermal hydrolysis treatment in wastewater treatment plants.

Mr. Stefan Sandbacka Vice President of Business Development at Cambi Group

The AMPTS II is a fantastic instrument, we use it constantly at Hanze University of Applied Sciences (The Netherlands), actually I have a presentation at the EUBCE in Stockholm where I will be presenting the talking about balancing the supply & demand of renewable Energy. Our AMPTS II has played a big part in providing us with a lot of reliable data…

Dr. Gert Hofstede Lecture at ALIFE- Research Centre Renewable Energy - ILST, Hanze University Groningen, The Netherlands saves time by enabling quick and automatic batch experiments with very little manual labour, and that it is “student proof”, because the instrument does not require long experience or training before you start using it.

Mr. Aurelien Perrault Project Manager, Sludge & Energy Innovation, Thames Water, UK

...the automation of the test minimises human errors, makes data collection more frequent than manual methods, and reduces operator time allowing more time for performing alternate tasks.

Associate Prof. Bernadette McCabe University of Southern Queensland, Australia

...the AMPTS II is the key piece of equipment for Digester Doc, as this equipment is used in determining current and potential energy values as well as in other troubleshooting activities. Digester Doc’s clients get help to identify the most suitable bacterial or mineral innoculants and feedstocks wisely, and potentially get at least 25% additional energy values out of their facilities. One of our many success stories is New Energy One, LLC in Idaho, where they were able to double production in just three months due to the AMPTS II usage.

Mr. Will Charlton President, Digester Doc, USA

...the AMPTS II significantly reduces labour demands and enables our students to more efficiently utilize time during class. The setup of the AMPTS II system is easy to follow and it's a powerful tool for demonstrating and teaching anaerobic digestion processes in both undergraduate and postgraduate courses.

Dr. Xundong Liu Tsinghua-SUEZ Environnement Environmental Science and Engineering Experiment and Practice Educational Laboratory, China

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